Gastric carcinoma and immunity - antibody are led and treated

Gastric carcinoma and immunity - antibody are led and treated
Ehrilich has proposed the imagination of leading and treating tumour with the antibody, and call it the evil spirit to play on beginning of this century. Come in a last one century, people are diligent constantly, test repeatedly, but this imagination has never been sent and realized very much. Since monoclonal antibody technology came out in 1976, people have lighted and employed the evil spirit to be played and treated the hope of tumour again, the reason is that antitumor monoclonal antibody is more peculiar, the antigen determines that it is clear to form a cluster, can produce in a large amount, may be more suitable to lead treating. Entered the eighties, our country conducted the preparation work of monoclonal antibody of gastric carcinoma on there are a lot of laboratories, it is very arduous work, can insist on perhaps. But some laboratories are from the preparation of monoclonal antibody of gastric carcinoma, to used in clinical diagnosis and treat finally, through making great efforts constantly, make very good achievement. If should realize the direction treatment of tumour, and fruitful, must pass the following several committed step. The preparing monoclonal antibody at present of monoclonal antibody prepares the method of taking cells to merge more. It adopts this method too to produce monoclonal antibody of gastric carcinoma both at home and abroad. But the intersection of person who want and specificity of getting well, the intersection of result and the intersection of price and high and the intersection of affinity and better antibody, must also take a lot of measures. Work hard in choosing the antigen at first. DaiMing, Zhang Xue at the monoclonal antibody MG series gastric carcinoma not prepared such as being mediocre Fan, prevent spending the cell department simply from, because the cell department is trained for a long time to always lose some antigen, and adopt the fresh sample and remedy this question. They combine the intersection of cell and department with fresh the intersection of gastric carcinoma and sample that operation excise, have illness coming on cell and the intersection of transformation and focal cell of cooking stove and spend originally, so apt to get monoclonal antibody that different antigen determines to form a cluster, therefore series MG received have more types. They prepared and screened a large number of antibodies at the same time, hybridized and chose in tumour cells carefully out several high-efficient prices from dozens of, high affinity, cell one secreted steadily, make up series MG. The second requisite measure is to choose a good screening antibody method. The commonly used screening method is that enzyme unites the immune absorption experiment (ELISA) And organize the chemistry method in immunity. Seen from the present, it is good later person, its advantage is to organize the antigenic composition in the section to be very complicated, antibody and any cell compositions react, once clear, such as monoclonal antibody of gastric carcinoma, it only should be the same as the cellular reaction of gastric carcinoma, if other cell compositions react, if does not non- peculiar dye, antigenic compositions cross, there is common antigen. Can lose the non- peculiar antibody dyed and reacted alternately, only leave the more peculiar antibody, so the result screened with the immune group is in an all-round way but completely, there is much information got. Organize in the method of chemistry in immunity, with ABC being sensitive in law, react weaker antibody can reveal out even. The fluorogenic indirect method of the immunity, it is very convenient to use too, but sensitiveness is a little worse, the two can save time. Have one the intersection of question and city should pay attention to when screening, it is paraffin wax that slice and not icy to slice under the withered sure situation using. Slice by paraffin wax at it screen antibody, can elect by result, the prices is high,can antibody dyed in paraffin wax section. But the paraffin wax slices the treatment passing alcohol and Xylene lipoprotein solvent, the lipoprotein antigen has already been removed, so the antibody related to lipid-soluble antigen can't be screened out. Must use the icy section while confirming antibody and those organizations react alternately, because icy sliced, the antigen has not been lost, except for react alternately, it is the real crossing, slice, find with paraffin wax antibody with organize, it reacts to be reliable while being other. Specific monoclonal antibody of monoclonal antibody can be employed in the human body validly, there must be higher specificity and affinity. Must be positive with the stomach cancer cell in most gastric carcinoma sample section at first. It is better if can the positive reaction arises to most cells and gastric carcinomas of different types among them. The mouse mouse antibody of series MG is 67%~100% to all kinds of positive rate of gastric carcinoma of splitting up, the cohesion to gastric carcinoma of PCI antibody is stronger. Another one it measures specific methods to be to inject with nuclide antibody into the naked mouse with people's gastric carcinoma behind the mark, observe whether relevant antibodies are concentrated in people's tumour. If to resist MGb2, combine behind the 131I only gastric carcinoma, give, carry on one's shoulder or back the intersection of people and the intersection of gastric carcinoma and naked mouse, inject, can apparent to look like outside body. At 7 day under injection, radiation that tumour organize normally than 16.22, prove the antibody can concentrate 16 times in gastric carcinoma. After can combine 131IMGb antibody to pour into at the same timing, tumour grows slowly, tumour presents large stretch of necrosis while organizing, and blank contrast, simple control group of antibody tumour fast-growing, split looks more but few necrosis. Can also hand in the antibody to unite anticancer drug outside the body, add into cultivation system of stomach cells, have stronger direction that wound function more than the simple medicine or simple antibody after in order to observe antibodies and combine medicines. To the detection of the cells wounded, released with Cr51 in the past, such methods as 3HTdr was mixed, multi-purpose thiazole was blue (MTT) now Method,last nuclide,complete,there are getting worse on accuracy. The natural antibody can be concentrated in the naked mouse which carries on one's shoulder or back people's tumour, so it is not obvious to wound tumour function. Because monoclonal antibody is produced from the mouse, the organization of the mouse does not react, only to lead tumour of people react, so this kind it concentrates to be natural, but when people use, of different people organization unavoidable to have, react alternately with people's gastric carcinoma on antigen, so it is much more difficult to concentrate. Certainly find the specific index of monoclonal antibody has much more, such as see mark antibody can be bound with ultra micro-structure of cancer cell of the stomach or shifted in cells peculiarly under the intersection of immunity and electric mirror ' Melt inside) ; Can also measure the affinity of the antibody. Monoclonal antibody employ to should spend a lot of monoclonal antibody in the human body, such as make immunity, organize reagent of chemistry with it, carry on pathological diagnosis, equip ELISA reagent box and diagnose etc. serology of gastric carcinoma with it. These are reported in gastric carcinoma monoclonal antibody of series MG and other series to some extent. But how is speak herein specific and good, can orient antibody of gastric carcinoma of people, use diagnosis of patient for and treat in carrying on one's shoulder or back naked mouse, people of tumour. The diagnosis and treat, known as leading to diagnosing and leading to and treating. Monoclonal antibody is used before the human body, must carry on some inspections: There is no heat source; Do acutely and poisonously and test, at the time of the little mouse tail intravenous injection such as giving, no death or pathological change,etc. will take place. And is tried by the volunteer, avoid the bad reaction, then can declare Ministry of Public Health and sanction carrying on clinical patient's application. Reach the national biologicals and appraise the standard of the department, is carried on for 2 stage in the designated hospital, after a clinical trial, can sanction on the market Sell. Series MG and series PC can be carried on apparently and looked like with the antibody of their marks of nuclide on patient at present, as the diagnosis of tumour, can also be used for carrying on the experiment treatment, but not made clear curative effect yet
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